In particular, cerebral arteries from obese animals show increased vasoconstriction in response to 5-hydroxtryptamine (5-HT), potassium chloride (KCl) and endothelin-1 (ET-1), and reduced vasodilation in response to acetylcholine.83,93,119 However, other authors reported no effect of high-fat diet on vascular responsiveness, or attributed it to co-morbid hypertension.79,82 These changes may have a functional impact on cerebral blood flow, for example obese mice show an altered response in cerebral blood flow after whisker stimulation.93,120. Here, EDN1 is linked to hypertensive disorder.