This well-characterised model [61] involved sensitisation of mice with a ‘low dose’ of the allergen OVA (1 μg) with Alum (0.2 mg), which caused methacholine-induced airway hyperresponsiveness, airway eosinophilia and neutrophilia, increases in BALF levels of IL-5, and circulating allergen-specific IgE and IgG [24]. Here, IGHE is linked to airway hyperresponsiveness.