Furthermore, LPS-induced sepsis reduced the level and frequency of Ca2+ events (TRPV4 sparklets and IP3-mediated Ca2+ pulsars) in the endothelium, as well as the vasodilator response to the muscarinic receptor agonist carbachol – both of which are indicators of endothelial dysfunction and dysregulation of blood flow22, 29, 31. This evidence concerns the gene TRPV4 and endothelial dysfunction.