Furthermore, Ding et al. showed that cardiac overexpression of SIRT1, a NAD+-dependent deacetylases, reduced diabetes-exacerbated myocardial ischemia reperfusion injury and oxidative stress in diabetic rats via eNOS activation and that such effect was mediated by increase of the eNOS phosphorylation and reduction of the eNOS acetylation [77]. This evidence concerns the gene NOS3 and diabetes mellitus.