By qualitative RT-PCR analysis we assessed the expression not only of the ESC marker genes (Nanog, Sox2, KLF4, LIN28A, and POU5F1) in OSA3, OSA3-CSCs, and OSA3-CSCs II (Figure 25), but also of the expression of the pluripotency marker gene, MYC, and of the other specific cancer stem cells marker genes (PROM1, ALDH1A1, CD34, EZR, and AXL) in OSA3 and in OSA3-CSC lines (Figure 26). This evidence concerns the gene POU5F1 and cancer.