Within the TCGA genomics data for pancreatic, lung [23, 24], head and neck [25], and colon tumors [26], mutations or copy number alterations of EGFR network genes are most pervasive in EGFR, RAS, and PI3K. Therefore, this study creates a cell line model of EGFR overexpression, mutant HRASV12D, and mutant PIK3CAH1047R in a controlled genetic background. Here, PIK3CB is linked to colonic neoplasm.