XDH and hyperuricemia: Herein, we conducted immobilized metal affinity chromatography and ultrafiltration-ultra performance liquid chromatography-mass spectrometry (IMAC-UF-UPLC-MS) in vitro investigations to identify the XO inhibitors rapidly and efficiently, in order to clarify the potential pharmaceutical basis for the use of G. hypoleucum in treating gout and hyperuricemia.