BRCA1 and non-small cell lung carcinoma: Powerful metrics are available to detect FANCD2/BRCA1 foci formation, such as the FA triple-staining immunofluorescence based method (FATSI), which identified a subset of non-small-cell lung cancer (NSCLC) tumors that were deficient in FANCD2/BRCA1 foci and thus were repair deficient [32].