In the stepwise regression model formed with risk factors such as age, sex, BMI, WBC, Hb, CRP, native thiol, total thiol, disulphide, disulphide/native thiol, disulphide/total thiol, and native thiol/total thiol, risk factors as disulphide/native thiol (OR = 1.368; p = 0.018) and CRP (OR = 1.635; p = 0.003) were determined as predictors of perforated appendicitis compared to the nonperforated group. This evidence concerns the gene GSTM1 and appendicitis.