In the present study, we developed a combined therapy, pHRE-Egr1-HSV-TK/131I-antiAFPMcAb-GCV/MFH, for hepatoma by using PEI-Mn0.5Zn0.5Fe2O4 nanoparticles (PEI-MZF-NPs) as magnetic media for MFH and gene transfer vector for gene-therapy, and investigated the therapeutic effects of pHRE-Egr1-HSV-TK/131I-antiAFPMcAb-GCV/MFH on hepatoma in vitro and in vivo, as well as the underlying safety. Here, EGR1 is linked to hepatocellular carcinoma.