APP and Alzheimer disease: In order to find out if gangliosides play a role in Aβ toxicity in vivo, Ugcgf/f//CamKCreERT2 mice [35] were bred to 5xFAD//Ugcgf/f mice (5xFAD mice) harboring five familial AD mutations (APP K670N/M671L (Swedish mutation), I716V (Florida mutation), V717I (London mutation), and presenilin1 M146L and L286V) [36].