In the present study, UTI pretreatment significantly blocked the secretion of MMP-9, RANK and cathepsin K in a dose-dependent manner compared with LPS/PMMA-stimulated cells, though various concentration of UTI (500 or 5000 units/ml) pretreatment showed a similar reduction in the case of TNF-α and IL-6. This evidence concerns the gene TNF and bacterial urinary tract infection.