FLT3 and acute myeloid leukemia: In FLT3/ITD+ AML, loss of heterozygosity of the wild type allele is sometimes observed, and is associated with poorer survival.[22–24] To investigate whether or not this phenomenon also occurred in our model, genomic DNA was extracted from bone marrow and matched tail tissue from leukemic mice, and PCR amplification was performed using primers flanking the ITD mutation (Figure 3A).