VCL and hydrops fetalis: Our BD/2D-GE approach had identified six VCL polypeptides (spot # 52, 54, 57, 58, 59, and 63; pI: 6.39–7.53) that were close to full-length protein in size (MW: 99–108 kDa) and decreased in abundance as well as S-NO modification levels in HF subjects (Table 1).