While in both subgroups divided by sample size, Gli-1 expression was correlated to high depth of invasion in gastric cancer patients (n ≤ 70: OR 6.92, 95%CI 1.72-27.88, P = 0.007; n > 70: OR 9.24, 95%CI 2.85-29.92, P = 0.000). Here, GLI1 is linked to gastric cancer.