In addition, our results showed that gene silencing of HDAC9 significantly decreased HG-induced podocytes injury in vitro, and attenuated renal injury through improving glomerulosclerosis, regulating apoptosis factors, increasing Nephrin and Podocin expression, and reducing proinflammatory cytokines and chemokines production in diabetic db/db mice. This evidence concerns the gene HDAC9 and glomerulosclerosis.