In agreement, as compared to WT mice, Mif-/- mice exhibited reduced serum AST (alanine aminotransferase, reflecting systemic tissue injury) and ALT (aspartate aminotransferase, reflecting liver injury) levels (Fig 1C and 1D), as well as reduced hepato- and splenomegaly that coincided with a lower increase in the number of white blood cells (WBC) in the liver and the spleen of Mif-/- mice (Figs 1E and 1F and S3). This evidence concerns the gene MIF and Splenomegaly.