So far, there are no specific pharmacological inhibitors or genetics-manipulated animals available, therefore we chose the widely used in vitro model of BBB (i.e., cultured endothelial monolayer) and the in vitro model of ischemia (i.e., OGD) to investigate whether ENOPH1 plays a role in ischemic BBB injury. The gene discussed is ENOPH1; the disease is ischemia.