We showed that BG-4 is very potent in inhibiting proliferation of both colon cancer cells, promoted apoptosis as measured by flow cytometry and microscopy study and the mechanism of action involved is through downregulation of antiapoptotic proteins Bcl-2 and XIAP, upregulation of proapoptotic proteins Bax and caspase-3 and modification of cell cycle proteins p21 and cyclin dependent kinase 2 (CDK2). This evidence concerns the gene CASP3 and colonic neoplasm.