CASP3 and malignant colon neoplasm: As shown in Fig. 6a, BG-4 at 125 μg/mL significantly modified the expression of different apoptotic markers in HCT-116 colon cancer cells by increasing the expression of Bax and caspase-3 by 1.3-fold and 2.2-fold, respectively and decreasing the protein expression of Bcl-2 and XIAP by 44.3% and 67.0%, respectively.