Then we detected the expression of STYK1 in human normal liver cell line LO2 and 5 HCC cell lines (7402, 7721, Hep3B, 97H, and LM3), and results demonstrated a higher expression of STYK1 in HCC cell lines, compared with LO2 (Fig. 1B). This evidence concerns the gene STYK1 and hepatocellular carcinoma.