CD40LG and infection: For the whole cell B. bacilliformis IgM ELISA, the 3 standard deviation cut off method resulted in 95.5% of volunteers presenting levels of IgM equivalent to evidence of infection, while with the use of FMM, the cut off was established at 0.35 (IgM) and 0.53 (IgG), with approximately 34 and 27% of volunteers showing evidence of infection by IgM and IgG values, respectively.