PC-3/N cells showed slightly decreased random cell movement in the absence of FBS; however directional cell migration induced by FBS was significantly down by ∼50% in PC-3/N cells compared to PC-3/C (p-value<0.001), indicating that NLGN4Y overexpression negatively impacted prostate cancer cell migration (Figure 8E). This evidence concerns the gene NLGN4Y and prostate cancer.