HSPB1 and melanoma: Our results demonstrated HSPB1 expression could be strikingly upregulated by hyperthermia (Figure 1A, D; Figure 5E), and knock down of HSPB1 further reduced cell viability and proliferation in hyperthermic treated melanoma cells when compared with either single agent alone (Figure 2A-C, Figure 7A-B), providing evidence that silencing of thermosensitive HSPB1 combined with hyperthermia could increase the sensitivity of melanoma cells to each agent and improve the overall impact on cell survival or cell proliferation.