MC4R and infection: After 3 days of renal PRV-614 infection (n = 3, phase I), colocalization of PRV-614 and MC4R-GFP immunopositive neurons was seen and largely confined to areas in the sympathetic preganglionic neurons (SPNs) in the ipsilateral intermediolateral cell column (IML) of the thoracic spinal cord (Figure 1).