VTCN1 and colorectal carcinoma: When we tested the effect of TGF-β1 on the expression of mRNA and protein of B7-H3 and B7-H4, we found that the effects of TGF-β1 on the expression of mRNA and protein of B7-H3 and B7-H4 were almost the same among CRC cell lines (Figure 2A, 2D and 2E).