Thus, in the present study we set out to determine the effects of (i) application of recombinant human TFF3, (ii) transient TFF3 overexpression and (iii) stable, lentiviral TFF3 overexpression on growth, viability, proliferation, apoptosis as well as anchorage-independent growth, migration and tumor formation capacity of different human retinoblastoma cell lines. Here, TFF3 is linked to retinoblastoma.