Nestin knockdown AN3CA and KLE cells exhibited downregulation of the early transcriptional mesenchymal markers (SNAIL, SLUG, and TWIST), cell adhesion molecule (N-cadherin), mesenchymal intermediate filament (vimentin) and upregulation of an epithelial marker, E-cadherin, which is the suppressor of tumor cell invasion and metastasis. The gene discussed is NES; the disease is neoplasm.