Likewise, we found that the expression levels of ITGA2, COL1A1 and COL11A1 in tumor tissues were obviously higher than non-tumor tissues (Figure S3B–S3D), which illustrates that tumor invasion and metastasis related pathways were up-regulated in tumor compared with non-tumor tissues, especially for FLNC.These results further demonstrated that the up-regulation of FLNC is a common phenomenon in HCC and FLNC might have the potential to be used as a biomarker for HCC diagnosis. The gene discussed is COL1A1; the disease is hepatocellular carcinoma.