LEP and liposarcoma: An experimental study investigating methylation-dependent transcriptional activity of a human LEP promoter fragment in Lisa-2 cultured cells (a liposarcoma cell line) showed that methylation of the CpG4 site (corresponding to CpG position –51 in Figure 6 of Melzner et al. 2002) was important for down-regulation of promoter activity of LEP (Melzner et al. 2002).