Western blots analysis showed that the protein levels of Wnt2 and Wnt3 were significantly decreased after CRS in the VH but not in the DH (Figure 1c; Wnt2, t16=5.175, P<0.001; Wnt3, t16=3.334, P=0.004, two-tailed t-test; Figure 1d; Wnt2, t16=0.766, P=0.455; Wnt3, t16=−0.213, P=0.834, two-tailed t-test). This evidence concerns the gene WNT3 and congenital rubella syndrome.