Previous studies have reported that transcription of both Wnt2 and Wnt3 is CREB dependent.47, 48 Cultured neurons (7 DIV) were infected with Lenti-Wnt2 or Lenti-Wnt3 virus for 3 days, and we observed that although the total CREB levels exhibited no significant alteration, the levels of p-CREB were significantly increased after Lenti-Wnt2 or Lenti-Wnt3 virus infection, which suggested that both Wnt2 and Wnt3 activated CREB (Figure 4c; F2,12=36.713, P<0.001, one-way ANOVA; post hoc test, Lenti-Wnt2, P<0.001; Lenti-Wnt3, P<0.001). Here, CREB1 is linked to viral infectious disease.