Further analyses of germaria and egg chambers by Tj and Vasa IF staining revealed various cellular defects due to the overexpression of somatic c-Fos (Fig 7D), such as abnormal cell organization in 57%–65% of germaria (Fig 7E), excessive and disorganized somatic cells in 10% –32% of germaria (Fig 7Di and 7F), and cyst accumulation (>3 cysts) in 44% of germaria (Fig 7Dii and 7G). This evidence concerns the gene DDX4 and cyst.