To further study whether p-IRAK1 (T209) contributes to HCC cells proliferation, the inhibitor of IRAK1/4, which selectively inhibits the activities of IRAK1 and IRAK4, was used to repress the activity of IRAK1 in SMMU-7721 and HepG2 cell lines. The gene discussed is IRAK4; the disease is hepatocellular carcinoma.