CD4 and cancer: Further characterization of all fifteen obtained peptide-stimulated LNMC cultures (both research and GMP), derived from cells initially expanded with 150 U/ml of IL-2, was performed by flow cytometry using an antibody panel for memory and inhibitory markers as well as to detect HPV-specific CD4+ CD25+ FoxP3+ regulatory T cells, for which we previously showed that they can be present in patients with HPV16-induced cancer [18, 28].