CTNNB1 and intrahepatic cholangiocarcinoma: Recent evidence has shown that CCA, another form of primary liver cancer, is dependent on the Wnt pathway despite the lack of CTNNB1 mutations in malignant cholangiocytes.27, 35–37 In order to understand if our findings were disease-specific or could be extended also to CCA, we looked at uc.158− expression in an initial cohort of British human intrahepatic CCAs (ICC) and found that uc.158− was significantly overexpressed in tumour tissues compared with healthy livers (figure 5A).