Our previous studies indicated that breast cancer cells that bear mesenchymal properties use the ARF6-based pathway for invasion and metastasis.20, 21, 24, 25, 28 Here, we show that the ARF6-based pathway possesses a mesenchymal property, in which AMAP1 binds to EPB41L5; and that EPB41L5 is primarily induced by ZEB1 during the breast cancer mesenchymal program triggered by various events. Here, ARF6 is linked to breast cancer.