ARHGEF5 and colorectal cancer: To elucidate the role of ARHGEF5 in tumor growth from human colorectal cancer cells, we examined the effects of ARHGEF5 KD on anchorage-independent cell growth in epithelial-like HCT116 and HT29 cells as well as in mesenchymal-like SW480 and SW620 cells (Figures 5a and b).