POU5F1 and hepatocellular carcinoma: BCLC9 cell line was established from a well-differentiated human HCC [12] and they show a stem cell phenotype characterized by gene and protein expression of a pool of pluripotency markers: OCT4, NANOG, SOX2, KLF4, CD133, EpCAM, KRT19 genes, and overexpression of MYC which is not due to MYC gene amplification (Supplementary Figure S1A–S1C).