To confirm the knockdown efficiency of the specific siRNAs, we first detected the endogenic expression level of KPNA2 and OCT4 in bladder cancer cell lines J82, T24, 5637, UM-UC-3 and human uroepithelial cell line SV-HUC-1 through RT-qPCR and Western-blot analysis. This evidence concerns the gene POU5F1 and urinary bladder cancer.