NTSR1 and hepatocellular carcinoma: Although an increase in Wnt5 expression was observed through PCR array and Western blot, the non-canonical Wnt/Ca2+ pathway was not considered as the vital signaling pathway regulating the NTS-mediated EMT in HCC because no detectable evidences of the PKC protein activation were observed after NTS stimulation was induced or NTR1 expression was increased.