We detected the expression of well-known EMT-related protein markers and some transcription factors (TFs), including E-cadherin, N-cadherin, β-catenin, Snail, and Slug, through RT-qPCR assay and Western blot to demonstrate how NTS+NTR1+ promoted HCC invasion and migration. Here, NTSR1 is linked to hepatocellular carcinoma.