Although an increase in Wnt5 expression was observed through PCR array and Western blot, the non-canonical Wnt/Ca2+ pathway was not considered as the vital signaling pathway regulating the NTS-mediated EMT in HCC because no detectable evidences of the PKC protein activation were observed after NTS stimulation was induced or NTR1 expression was increased. This evidence concerns the gene PRRT2 and hepatocellular carcinoma.