We compared the expression levels of all genes in these three pathways among differently NTS-responsible HCC cell lines and found that most differentially expressed genes were concentrated in the Wnt pathway, including CTNNB1, WNT5A, WNT 5B, and WNT11, which remarkably increased by more than twofold after NTS stimulated. Here, WNT5A is linked to hepatocellular carcinoma.