H&E and immunohistochemical analysis of the ablation zone after laser ablation demonstrated discrete morphologic regions within the ablation zone that correlated with TTC vital staining (Fig 4D) and markedly increased immunostaining for phospho-AKT (Fig 4F) and phospho-ERK (Fig 4H) at the tumor ablation margin but not at distances further from the tumor ablation margin or at the liver ablation margin (S7 Fig for phospho-AKT). This evidence concerns the gene MAPK1 and neoplasm.