To investigate whether GPR30 diminished the function of GABAergic interneurons during the development of BCP, RNAi-LV targeting GPR30 was injected into the ispilateral dorsal horn of cancer-bearing rats, the localization of GPR30 on GABAergic neurons was analyzed using inmmunofluorescent double staining with antibodies of GPR30 and GAD67 (a marker of GABAergic interneuron). The gene discussed is GAD1; the disease is cancer.