GPER1 and cancer: To investigate whether GPR30 diminished the function of GABAergic interneurons during the development of BCP, RNAi-LV targeting GPR30 was injected into the ispilateral dorsal horn of cancer-bearing rats, the localization of GPR30 on GABAergic neurons was analyzed using inmmunofluorescent double staining with antibodies of GPR30 and GAD67 (a marker of GABAergic interneuron).