CD34 and acute myeloid leukemia: LSCs were previously characterized to be exclusively within the c-kit+Sca-1− population in this model of MLL-AF9-driven AML, where CD16/32 is highly enriched but CD34 expression is dispensable.2, 24 Data from our laboratory has shown that the c-kit+Sca-1− population of MLL-AF9-induced AML cells is almost exclusively CD16/32+ and CD34− (unpublished observations) and we therefore refer to these cells as phenotypic LSCs.