It is perhaps worth noting that the use of a single-protein ARL4C to be subtype II marker and its use in classifying tumours as subtype II or not, using immunohistochemistry had quite a significant error rate (31% of their tumour samples were misclassified into a different subtype than the original classification based on gene expression profiles), so perhaps a combination of markers would help to improve the accuracy of an immunohistochemical classification. This evidence concerns the gene ARL4C and neoplasm.