Experimental limitations prohibited a comprehensive study of both aspects in our model: Perforin-/- mice showed no difference in bacteremia, but increased organ loads at day 11 p.i. However, according to ethical guidelines, mice had to be sacrificed between days 10 and 13 p.i., i.e. before the onset of measurable liver injury in control mice (S4 Fig). The gene discussed is PRF1; the disease is bacterial infectious disease with sepsis.