EZH2 and infection: To test which domains of LANA are important for the recruitment of LANA and EZH2 to the KSHV promoters during de novo infection, we constructed lentiviruses expressing 3xFLAG-tagged LANA WT or point mutants that are deficient in either DNA or chromatin binding activity, and used them to complement LANA deficiency in LANA/RTA dKO KSHV-infected cells (Fig 7A and 7B).