To investigate the biological functions of the different biochemical properties of the 2b protein in the context of CMV infection in plants, in this study, we further created mutations of 2b of the genomic RNA2 according to the two main biochemical properties of double-stranded RNA (dsRNA) and AGO binding activities (Duan et al., 2012). The gene discussed is FBXW7; the disease is cytomegalovirus infection.