Although we found that the endogenous HIPSTR gene is not expressed in NT2/D1 embryonal carcinoma cells, in this pluripotent cell line two HIPSTR promoter-luciferase constructs (pGL3-P1 and pGL3-P3) produced ~35–50-times stronger luminescence signal than did positive control construct (pGL3-SV40) (Fig. S2B). Here, TFAP2A-AS2 is linked to embryonal carcinoma.