CAMK2G and breast cancer: Furthermore, MDA-MB-231 and MCF-7 cells overexpressing the T286D phosphomimic mutant form of CaMKII (Fig. 4A,B) migrated significantly more rapidly than the WT and T286V phosphonull forms of CaMKII, indicating that phosphorylation of CaMKII at T286 further increases the migratory capacity of breast cancer cells.