The dual-luciferase reporter assay in this study displayed that NF-κB1 expressions were modulated by miR-9 in chondrocytes, which was consistent with results drawn from uveal melanoma cells, ovarian cancer cells, and gastric adenocarcinoma cells.[14,35–37] Furthermore, our study discovered that miR-9 mimics suppressed the NF-κB1 protein expression level in knee OA chondrocytes and the downregulation of miR-9 could trigger an increase in NF-κB1 expressions occurred at both gene and transcription levels in chondrocytes. The gene discussed is NFKB1; the disease is gastric adenocarcinoma.