PTEN and posterior cortical atrophy: Moreover, in contrast to 5 μM enzalutamide, which failed to inhibit spheroid growth, the PI3K inhibitor LY294002 attenuated LNCaP and even LNCaP/CAF spheroid growth, suggesting that upregulation of E-cadherin together with elevated activation of Akt is critical for growth and survival of PTEN-negative PCa spheroids, driving the cells to higher resistance towards anti-androgens.